BIO 675 RNA Next Generation Sequencing

This five day practical course consists of a library preparation starting from total RNA, followed by a sequencing run on a bench top sequencer. An introduction to the analyses of the resulting data and some exercises will be offered too. The lectures cover existing and upcoming NGS technologies, their applications and the principles of downstream data analysis
The data analysis part starts off from the raw reads, as generated by the instrument, and cover pre-​processing, alignment, several quality control checkpoints, transcripts expression quantification and differential expression analysis, and comparison of bulk RNA-​seq versus single cell RNA-​seq.
By the end of the course participants should be able to make informed decisions about which technology and workflow to apply to solve specific research questions.